Revealing Restriction Enzyme Dynamics With Fast-scan Afm
نویسندگان
چکیده
منابع مشابه
Fast-scan AFM observations of restriction enzyme
The excision and repair of mismatched base pairs is an essential task of cell maintenance. Several models have been proposed to describe how the hemimethylated d(GATC) sequence can direct Escherichia coli mismatch repair while residing on either side of a mismatch, at a separation distance of 1,000 bp or more. Anna Pluciennik and Paul Modrich report that a protein roadblock and breaks in the DN...
متن کاملRevealing time bunching effect in single-molecule enzyme conformational dynamics.
In this perspective, we focus our discussion on how the single-molecule spectroscopy and statistical analysis are able to reveal enzyme hidden properties, taking the study of T4 lysozyme as an example. Protein conformational fluctuations and dynamics play a crucial role in biomolecular functions, such as in enzymatic reactions. Single-molecule spectroscopy is a powerful approach to analyze prot...
متن کاملFast-scan atomic force microscopy reveals that the type III restriction enzyme EcoP15I is capable of DNA translocation and looping.
Many DNA-modifying enzymes act in a manner that requires communication between two noncontiguous DNA sites. These sites can be brought into contact either by a diffusion-mediated chance interaction between enzymes bound at the two sites, or by active translocation of the intervening DNA by a site-bound enzyme. EcoP15I, a type III restriction enzyme, needs to interact with two recognition sites ...
متن کاملAdaptive AFM scan speed control for high aspect ratio fast structure tracking.
Improved imaging rates in Atomic Force Microscopes (AFM) are of high interest for disciplines such as life sciences and failure analysis of semiconductor wafers, where the sample topology shows high aspect ratios. Also, fast imaging is necessary to cover a large surface under investigation in reasonable times. Since AFMs are composed of mechanical components, they are associated with comparably...
متن کاملCantilever dynamics in AFM
Dynamic atomic force microscopy, in essence, consists of a vibrating microcantilever with a nanoscale tip that interacts with a sample surface via shortand long-range intermolecular forces. Microcantilevers possess several distinct eigenmodes and the tip-sample interaction forces are highly nonlinear. As a consequence, cantilevers vibrate in interesting, often unanticipated ways; some are detri...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2009
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2008.12.2013